Supporters say couples plan to line up before the city's marriage bureau opens at 8:30 a.m. Wednesday, and court officials have been told to expect up to 200 people.
Washington is now the sixth place in the nation where gay marriages can take place. Connecticut, Iowa, Massachusetts, New Hampshire and Vermont also issue licenses to same-sex couples.
Because of a mandatory waiting period, couples won't actually be able to marry in the District of Columbia until March 9.
The D.C. Council passed a bill in December that allows gay couples to wed. The bill then underwent a congressional review period, which ended Tuesday.
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